[Background]\ud\udBacteroides fragilis is the most frequent species at the human intestinal mucosal surface, it contributes to the maturation of the immune system although is also considered as an opportunistic pathogen. Some Bifidobacterium strains produce exopolysaccharides (EPS), complex carbohydrate polymers that promote changes in the metabolism of B. fragilis when this microorganism grows in their presence. To demonstrate that B. fragilis can use EPS from bifidobacteria as fermentable substrates, purified EPS fractions from two strains, Bifidobacterium longum E44 and Bifidobacterium animalis subsp. lactis R1, were added as the sole carbon source in cultures of B. fragilis DSMZ 2151 in a minimal medium. Bacterial counts were determined during incubation and the evolution of organic acids, short chain fatty acids (SCFA) and evolution of EPS fractions was analysed by chromatography.
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机译：[背景] \ ud \ ud脆弱类杆菌是人类肠道粘膜表面上最常见的物种，尽管也被认为是机会病原体，但它有助于免疫系统的成熟。一些双歧杆菌菌株会产生胞外多糖（EPS），这是一种复杂的碳水化合物聚合物，当这种微生物在它们的存在下生长时，它们会促进脆弱脆弱芽孢杆菌的代谢变化。为了证明脆弱的芽孢杆菌可以使用双歧杆菌的EPS作为可发酵底物，从两个菌株长双歧杆菌E44和动物双歧杆菌亚种中纯化得到的EPS馏分。在基本培养基中，添加了乳酸R1作为脆弱芽孢杆菌DSMZ 2151培养物中的唯一碳源。在孵育过程中确定细菌计数，并通过色谱分析有机酸，短链脂肪酸（SCFA）的演变和EPS组分的演变。
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